Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Cytogenetic : Conventional Versus Modern Technology "
Presenter : Mrs Norita Salim &Mrs Juriah Togeman
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 23 December 2009, Wednesday
Time : 12.45 noon
Refreshment will be provided.
All are invited.
Thank you.
Tuesday, December 22, 2009
Monday, December 14, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " A Comparative Analysis of The Serum Proteome
of Oral Cancer Patients "
Presenter : Mr. Mohd Yusri Idorus
Chairperson : Mr. Abu Thalhah Abdul Aziz
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 16 December 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " A Comparative Analysis of The Serum Proteome
of Oral Cancer Patients "
Presenter : Mr. Mohd Yusri Idorus
Chairperson : Mr. Abu Thalhah Abdul Aziz
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 16 December 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, December 7, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Live Cell Imaging of Normal Human Osteoblast Cells using confocal Laser
Scanning Microscope
Presenter : Ms. Salina Othman
Chairperson : Mrs. Norita Salim
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 9th December 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, November 30, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : “Antioxidant and antiproliferative properties of Murraya Koenigii leaves extracts
and essential oil. “
Presenter : Mrs. Wan Nor I’zzah Wan Mohd Zain
Chairperson : Ms. Zuraini Dollah
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 2nd December 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
Monday, November 23, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Development of A Cryo SEM Method for Ultrastructural Investigation of Murine Embryos at Different Developmental Stages."
Presenter : Mr. Mohd Shahir Abdul Rahman
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 25 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Mr. Mohd Shahir Abdul Rahman
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 25 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, November 16, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " A time course study on the Effect of restraint stress exposure on the embryos development mice."
Presenter : Dr. Nasibah Azme
Chairperson : Mr. Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 18 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Dr. Nasibah Azme
Chairperson : Mr. Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 18 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, November 9, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " The Effects of Vitamin E supplementation on Bone Proteomics in Ovariectomised rats"
Presenter : Mr. Patrick N. Okechukwu
Chairperson : Mr. Mohd Yusri Idorus
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 11 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Mr. Patrick N. Okechukwu
Chairperson : Mr. Mohd Yusri Idorus
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 11 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, November 2, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : “Phylogenetic relationship of Nontypeable Heamophilus influenza isolated in Malaysia."
Presenter : Ms. Nurul Hamirah Kamsani
Chairperson : Mr. Mohd Yusri Idorus
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 4 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : “Phylogenetic relationship of Nontypeable Heamophilus influenza isolated in Malaysia."
Presenter : Ms. Nurul Hamirah Kamsani
Chairperson : Mr. Mohd Yusri Idorus
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 4 November 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, October 26, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Networking of differentially expressed genes in human cancer cells resistant to methotrexate."
Presenter : Mr. Mohd Ros Sidek
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 28 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Mr. Mohd Ros Sidek
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 28 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, October 19, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Elucidation of Cryotolerance in murine preimplantation embryos using confocal microscopy and development studies.
Presenter : Mr. Razif Dasiman
Chairperson : Mrs. Nor Shahida Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 21 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Mr. Razif Dasiman
Chairperson : Mrs. Nor Shahida Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 21 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, October 12, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Aminal Handling Technique"
Presenter : Mrs. Yuhaniza Shafinie Kamsani
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 14 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Aminal Handling Technique"
Presenter : Mrs. Yuhaniza Shafinie Kamsani
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 14 October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, October 5, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Effect of palm Oil Tocotienol (TriE) on Lipid profilr Enzime Antioxidant and Oxidative Stress Among Healthy Older Adults Individual. "
Presenter : Mr. Mohammad Johari Ibrahim
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 7th October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "
Presenter : Mr. Mohammad Johari Ibrahim
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 7th October 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Saturday, October 3, 2009
WORKSHOP Announcement
Whole genome based association studies for SNP and CNV based approaches
Research Instruments in conjunction with the Institute of Medical Molecular Biotechnology and Faculty of Medicine invites you to our workshop:
Venue: Institute for Medical Molecular Biotechnology (IMMB)
Lecture Hall 1, Level 5, Faculty of Medicine,
Tower 1, Science & Technology Complex
University Technologi MARA (UiTM) Shah Alam
Date: 12 October 2009
Time: 9.00am – 5.30pm
Research Instruments in conjunction with the Institute of Medical Molecular Biotechnology and Faculty of Medicine invites you to our workshop:
Venue: Institute for Medical Molecular Biotechnology (IMMB)
Lecture Hall 1, Level 5, Faculty of Medicine,
Tower 1, Science & Technology Complex
University Technologi MARA (UiTM) Shah Alam
Date: 12 October 2009
Time: 9.00am – 5.30pm
Friday, October 2, 2009
NEWS - Scientist at Berkley Lab decipher missing piece of first-responder DNA repair machine.
Scientist at Berkeley Lab decipher missing piece of first-responder DNA repair machine.
BERKELEY, CA — Scientists from the U.S. Department of Energy’s Lawrence Berkeley National Laboratory and the Scripps Research Institute have uncovered the role played by the least-understood part of a first-responder molecule that rushes in to bind and repair breaks in DNA strands, a process that helps people avoid cancer.
for more info follow the link below:
http://newscenter.lbl.gov/press-releases/2009/10/01/dna-repair-uncovered/
BERKELEY, CA — Scientists from the U.S. Department of Energy’s Lawrence Berkeley National Laboratory and the Scripps Research Institute have uncovered the role played by the least-understood part of a first-responder molecule that rushes in to bind and repair breaks in DNA strands, a process that helps people avoid cancer.
for more info follow the link below:
http://newscenter.lbl.gov/press-releases/2009/10/01/dna-repair-uncovered/
Do you want to get your papers publish?
Genome Technology - October 2009
The Journal Perspective
By Meredith Salisbury
All scientists are well-versed in the "publish or perish" fear — but what about when publishing a paper is so difficult that you feel like the very process may do you in? Researchers looking to get their results into a highly regarded journal for all the world to see have no easy task ahead of them. One way to get through the process unscathed: get familiar with the journals you're interested in, and make friends with editors.
Before you do anything with that manuscript you're sitting on, take a few minutes to peruse the websites of the journals you're thinking about submitting to. They'll all have a page with information for authors; this can help you make sure your paper matches the scope of the journal, and it also includes helpful guidelines about formatting and other details of the submission process. Ignore these at your peril: formats are chosen for a reason, and failing to conform to them can send a couple of bad messages — that you simply don't care enough to check guidelines, or that your manuscript was formatted for another journal and that wherever you're sending it this time wasn't your first choice. Also, pay attention to data requirements; different journals (especially ones that are open access) can have different rules about where data must be deposited for the paper to be published.
Once you're familiar with the guidelines, it can be worthwhile to contact an editor at the journal with a simple note to gauge interest level. "It can be good to send a pre-submission inquiry," says Clare Garvey, editor at Genome Biology, who adds that these inquiries are usually answered very quickly. It's a simple, fast way to see if the paper fits well with the research scope of the journal.
When you do send your manuscript in, be sure the cover letter is tailored to the publication you're targeting. Garvey says a common mistake she sees is sending letters intended for other journals that have been copied and pasted on a paper that was perhaps sent somewhere else first.
If your manuscript is sent out for review and accepted, you're in great shape — and interactions with the journal should be straightforward. But what happens if the paper is rejected — or, worse, rejected without review? While most scientists will beat themselves up or fume over this, very few feel comfortable following up on it. Garvey says it's quite acceptable to contact the editor to say you're not happy with the decision and ask for advice or feedback. "We're perfectly open to being called or emailed" when this happens, she says. "If we can help authors by being as transparent as we can, then that's good for everyone."
Make new friends
The time to think about publishing is not just when you have a manuscript ready to go, though. You'll be in better shape to submit a paper or to ask an editor about a review decision if you've already gotten to know the editor ahead of time. Garvey says that conferences are a great place to take this step — editors routinely attend meetings, and it's their job to get acquainted with scientists in the field and get a sense of the latest research.
Because editors have a broad perspective on research across many fields, Garvey says it's important to remember that they can be "a huge resource" to bounce research ideas off. Editors might "even suggest additional experiments that might help that paper get into that journal," she adds.
And if you're not getting out to conferences as much as you'd like, Garvey says outreach is considered so important that some journals even send editors on lab visits when there's enough interest in how the editorial process works.
The Journal Perspective
By Meredith Salisbury
All scientists are well-versed in the "publish or perish" fear — but what about when publishing a paper is so difficult that you feel like the very process may do you in? Researchers looking to get their results into a highly regarded journal for all the world to see have no easy task ahead of them. One way to get through the process unscathed: get familiar with the journals you're interested in, and make friends with editors.
Before you do anything with that manuscript you're sitting on, take a few minutes to peruse the websites of the journals you're thinking about submitting to. They'll all have a page with information for authors; this can help you make sure your paper matches the scope of the journal, and it also includes helpful guidelines about formatting and other details of the submission process. Ignore these at your peril: formats are chosen for a reason, and failing to conform to them can send a couple of bad messages — that you simply don't care enough to check guidelines, or that your manuscript was formatted for another journal and that wherever you're sending it this time wasn't your first choice. Also, pay attention to data requirements; different journals (especially ones that are open access) can have different rules about where data must be deposited for the paper to be published.
Once you're familiar with the guidelines, it can be worthwhile to contact an editor at the journal with a simple note to gauge interest level. "It can be good to send a pre-submission inquiry," says Clare Garvey, editor at Genome Biology, who adds that these inquiries are usually answered very quickly. It's a simple, fast way to see if the paper fits well with the research scope of the journal.
When you do send your manuscript in, be sure the cover letter is tailored to the publication you're targeting. Garvey says a common mistake she sees is sending letters intended for other journals that have been copied and pasted on a paper that was perhaps sent somewhere else first.
If your manuscript is sent out for review and accepted, you're in great shape — and interactions with the journal should be straightforward. But what happens if the paper is rejected — or, worse, rejected without review? While most scientists will beat themselves up or fume over this, very few feel comfortable following up on it. Garvey says it's quite acceptable to contact the editor to say you're not happy with the decision and ask for advice or feedback. "We're perfectly open to being called or emailed" when this happens, she says. "If we can help authors by being as transparent as we can, then that's good for everyone."
Make new friends
The time to think about publishing is not just when you have a manuscript ready to go, though. You'll be in better shape to submit a paper or to ask an editor about a review decision if you've already gotten to know the editor ahead of time. Garvey says that conferences are a great place to take this step — editors routinely attend meetings, and it's their job to get acquainted with scientists in the field and get a sense of the latest research.
Because editors have a broad perspective on research across many fields, Garvey says it's important to remember that they can be "a huge resource" to bounce research ideas off. Editors might "even suggest additional experiments that might help that paper get into that journal," she adds.
And if you're not getting out to conferences as much as you'd like, Garvey says outreach is considered so important that some journals even send editors on lab visits when there's enough interest in how the editorial process works.
Monday, September 7, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Radiation Protection Procedures
Presenter : Mr. Tuan Muhd Hafiidz Tuan Kamauzaman
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 9th September 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Radiation Protection Procedures
Presenter : Mr. Tuan Muhd Hafiidz Tuan Kamauzaman
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 9th September 2009, Wednesday
Time : 12.45 noon
All are invited.
Thank you.
Monday, August 24, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Skim Akreditasi Makmal Malaysia (SAMM)
Presenter : Miss Zuraini Dollah
Chairperson : Miss Nurul Ashikin Mohamed Rawi
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 26 August 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : " Skim Akreditasi Makmal Malaysia (SAMM)
Presenter : Miss Zuraini Dollah
Chairperson : Miss Nurul Ashikin Mohamed Rawi
Venue : IMMB Meeting Room, Level 13, Block 5, S&T
Building
Date : 26 August 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
Wednesday, August 19, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : The Beneficial Experiences Obtained Through 15th weeks of Internship
Presenter : Mohd- Al' Hafiz bin Ab Halim
Title : Antioxidant Activity and Cytotoxicity Potential of Tinospora Crispa
Presenter : Nurul Ashikin bt Zainurdin
Title : A study on Atherosclerotic Lession Development from Rabbit Aorta by Molecular and Histomorphology
Presenter : Noraisah bt Majiri
Title : Effect of UV Light on Pseudomonas Aeruginosa
Presenter : Vaneesha a/p Vasudarajan
Title : Effect of UV Light on Staphylococcus
Presenter : Nor Marina bt Mohamed
Chairperson : M r. Mohd Shahir Ab Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 19th August 2009, Wednesday
Time : 12.30 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : The Beneficial Experiences Obtained Through 15th weeks of Internship
Presenter : Mohd- Al' Hafiz bin Ab Halim
Title : Antioxidant Activity and Cytotoxicity Potential of Tinospora Crispa
Presenter : Nurul Ashikin bt Zainurdin
Title : A study on Atherosclerotic Lession Development from Rabbit Aorta by Molecular and Histomorphology
Presenter : Noraisah bt Majiri
Title : Effect of UV Light on Pseudomonas Aeruginosa
Presenter : Vaneesha a/p Vasudarajan
Title : Effect of UV Light on Staphylococcus
Presenter : Nor Marina bt Mohamed
Chairperson : M r. Mohd Shahir Ab Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 19th August 2009, Wednesday
Time : 12.30 noon
All are welcome. Refreshment will be provided.
Thank you.
Monday, August 10, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Immuneresponse to Heamophilus Influenza Type B Infection
Presenter : Ms. Nurul Asyikin Mohd Rawi
Chairperson : M s. Noraisah Majiri
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 12 August 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Immuneresponse to Heamophilus Influenza Type B Infection
Presenter : Ms. Nurul Asyikin Mohd Rawi
Chairperson : M s. Noraisah Majiri
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 12 August 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Monday, August 3, 2009
Journal Club/ Seminar
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to inform you that IMMB weekly journal club tomorrow will be held as follow:
Title : "Widescreen Assay for the Multiplex Detection and
Quantitation of Cell Signaling Proteins and Serum
Biomarkers"
Presenter : Ms Anita Wan (Msc)
Application & Sales Manager (Asia-Pacific region)
EMD Chemicals, San Diego, USA
Venue : Lecture Hall Level 6, Block 2, Faculty of Medicine,
UiTM
Date : 5th August 2009, Wednesday
Time : 2.45pm - 4.45pm
I am pleased to inform you that IMMB weekly journal club tomorrow will be held as follow:
Title : "Widescreen Assay for the Multiplex Detection and
Quantitation of Cell Signaling Proteins and Serum
Biomarkers"
Presenter : Ms Anita Wan (Msc)
Application & Sales Manager (Asia-Pacific region)
EMD Chemicals, San Diego, USA
Venue : Lecture Hall Level 6, Block 2, Faculty of Medicine,
UiTM
Date : 5th August 2009, Wednesday
Time : 2.45pm - 4.45pm
Monday, July 27, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Multiplex Branch DNA for Parallel Quantitative Gene
Expression Profiling
Presenter : Mrs . Norita Salim
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 29th July 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Multiplex Branch DNA for Parallel Quantitative Gene
Expression Profiling
Presenter : Mrs . Norita Salim
Chairperson : Ms. Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 29th July 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Monday, July 20, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Role of miRNA in Hematopoiesis stem cell development
Presenter : Mrs Sharifah Izwan Binti Tuan Othman
Chairperson : Mrs Norita Binti Salim
Venue : Level 13, IMMB
Date : 22nd July 2009, Wednesday
Time : 12.45noon - 1.30pm
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : Role of miRNA in Hematopoiesis stem cell development
Presenter : Mrs Sharifah Izwan Binti Tuan Othman
Chairperson : Mrs Norita Binti Salim
Venue : Level 13, IMMB
Date : 22nd July 2009, Wednesday
Time : 12.45noon - 1.30pm
All are welcome. Refreshment will be provided.
Thank you.
Monday, July 13, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Scanning Probe Microscopy: An Essential Platform for Full Spectral Measurements in Life Science "
Presenter : Dr. Sun/ Mun Kok Lim
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 15th July 2009, Wednesday
Time : 12.30noon
All are welcome. Refreshment will be provided.
Thank you
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Scanning Probe Microscopy: An Essential Platform for Full Spectral Measurements in Life Science "
Presenter : Dr. Sun/ Mun Kok Lim
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 15th July 2009, Wednesday
Time : 12.30noon
All are welcome. Refreshment will be provided.
Thank you
Monday, July 6, 2009
IMMB Workshop
Dear IMMB Fellow, IMMB staff, Postgraduate Students & RAs,
Title: REAL TIME PCR WORKSHOP ON SNPs GENOTYPING APPLICATION
SPEAKER : MS SRINDA NATHAN, APPLIED BIOSYSTEM APPLICATION SPECIALIST
DATE : 9 -10 JULY 2009.
VENUE : LECTURE SESSIONS AT IMMB MEETING ROOM, LEVEL 13 IMMB
HANDS-ON SESSION AT LEVEL 5, IMMB LAB.
Thank you.
Title: REAL TIME PCR WORKSHOP ON SNPs GENOTYPING APPLICATION
SPEAKER : MS SRINDA NATHAN, APPLIED BIOSYSTEM APPLICATION SPECIALIST
DATE : 9 -10 JULY 2009.
VENUE : LECTURE SESSIONS AT IMMB MEETING ROOM, LEVEL 13 IMMB
HANDS-ON SESSION AT LEVEL 5, IMMB LAB.
Thank you.
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "TaqMan Chemistry for SNP Assay"
"HRM Method"
" When to Use One Over Another"
Presenter : Ms. Srinda Nathan, Analisa Scientific
Chairperson : Nor Shahida Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 9th July 2009, Thursday
Time : 12.00noon- 1.00pm
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "TaqMan Chemistry for SNP Assay"
"HRM Method"
" When to Use One Over Another"
Presenter : Ms. Srinda Nathan, Analisa Scientific
Chairperson : Nor Shahida Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 9th July 2009, Thursday
Time : 12.00noon- 1.00pm
All are welcome. Refreshment will be provided.
Thank you.
Monday, June 29, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "MACS Technology Overview "
Presenter : Angelica Lim (Canvio Sdn Bhd)
Chairperson : Tuan Muhd Hafiidz Tuan Kamauzaman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 1st July 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "MACS Technology Overview "
Presenter : Angelica Lim (Canvio Sdn Bhd)
Chairperson : Tuan Muhd Hafiidz Tuan Kamauzaman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 1st July 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Wednesday, June 24, 2009
Talk
Dear All,
Please be informed that IMMB will be arranging a short talk this friday.
Details as below:
Date: 26th June 2009
Time: 11am
Venue: IMMB level 13
Topic: qPCR -"what normalizer should I choose"
-"How do I determine biological significant changes"
Presenter: Dr Hartmut from Lonza
All are welcome.
Thank you.
Please be informed that IMMB will be arranging a short talk this friday.
Details as below:
Date: 26th June 2009
Time: 11am
Venue: IMMB level 13
Topic: qPCR -"what normalizer should I choose"
-"How do I determine biological significant changes"
Presenter: Dr Hartmut from Lonza
All are welcome.
Thank you.
Monday, June 22, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "LIVE CELL IMAGING OF NORMAL CELL LINES BY USING CONFOCAL LASER SCANNING MICROSCOPE (CLSM)"
Presenter: MR. MOHD ABDUL HALIM BIN ABDUL LATIF
Title: "GROWTH RATE PATTERN OF NORMAL HUMAN OSTEOBLAST"
Presente: MS. KHAIRUN NISA BT IBRAHIM ASRI
Title: "THE DETERMINATION OF ANTIMICROBIAL PROPERTIES FROM TRADITIONAL REMEDIES AGAINST MRSA"
Presenter : MS. MIMI AZUANA BT WAGIMON
Title : "ANTIOXIDANT AND ANTICANCER ACTIVITY OF EUGENIA AROMATICUM AND TINOSPORA CRISPA"
Presenter: MS. SITI NUR SHAFINAS BT SHAIKH ABD RAHMAN
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue: IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 24th June 2009, Wednesday
Time : 12.30 noon
All are welcome.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "LIVE CELL IMAGING OF NORMAL CELL LINES BY USING CONFOCAL LASER SCANNING MICROSCOPE (CLSM)"
Presenter: MR. MOHD ABDUL HALIM BIN ABDUL LATIF
Title: "GROWTH RATE PATTERN OF NORMAL HUMAN OSTEOBLAST"
Presente: MS. KHAIRUN NISA BT IBRAHIM ASRI
Title: "THE DETERMINATION OF ANTIMICROBIAL PROPERTIES FROM TRADITIONAL REMEDIES AGAINST MRSA"
Presenter : MS. MIMI AZUANA BT WAGIMON
Title : "ANTIOXIDANT AND ANTICANCER ACTIVITY OF EUGENIA AROMATICUM AND TINOSPORA CRISPA"
Presenter: MS. SITI NUR SHAFINAS BT SHAIKH ABD RAHMAN
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue: IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 24th June 2009, Wednesday
Time : 12.30 noon
All are welcome.
Thank you.
Monday, June 15, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "APPLICATION OF BIOANALYZER”
Presenter : Mrs. Norita Salim
Chairperson : Ms. Zuraini Dollah
Venue : IMMB Meeting Room, Level 13, Block 5,
Date :
Time :
All are welcome.
Thank you.
Tuesday, June 9, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "PRINCIPLES OF CONFOCAL LASER SCANNING MICROSCOPE (CLSM)"
Presenter : Ms. Rachel Lim Shu Fen (Hi-Tech Instruments Sdn. Bhd)
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 10th June 2009, Wednesday
Time : 12.30 noon
All are welcome.
Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "PRINCIPLES OF CONFOCAL LASER SCANNING MICROSCOPE (CLSM)"
Presenter : Ms. Rachel Lim Shu Fen (Hi-Tech Instruments Sdn. Bhd)
Chairperson : Mr. Mohd Shahir Abd Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 10th June 2009, Wednesday
Time : 12.30 noon
All are welcome.
Refreshment will be provided.
Thank you.
Monday, June 1, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "PRINCIPLE Of SCANNING ELECTRON MICROSCOPE"
Presenter : Mr. Mohd Shahir Abd Rahman
Chairperson : Mr. Mohd Faeez Sarulan
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 3rd June 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "PRINCIPLE Of SCANNING ELECTRON MICROSCOPE"
Presenter : Mr. Mohd Shahir Abd Rahman
Chairperson : Mr. Mohd Faeez Sarulan
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 3rd June 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Monday, May 25, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "COPY NUMBER VARIATIONS (CNV)"
Presenter : Dr Hoh Boon Peng
Chairperson : Mr. Mohamad Ros Sidek
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 27th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "COPY NUMBER VARIATIONS (CNV)"
Presenter : Dr Hoh Boon Peng
Chairperson : Mr. Mohamad Ros Sidek
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 27th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Sunday, May 17, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "The Bender MedSystems FlowCytomix Technology for Multiple
Analyte Detection and Easy Data Analysis with FlowCytomixPro Software
Presenter : Dr Birgit Osterhoff (International Distributor Coach)
Chairperson : Mrs Norita Salim
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 20th May 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "The Bender MedSystems FlowCytomix Technology for Multiple
Analyte Detection and Easy Data Analysis with FlowCytomixPro Software
Presenter : Dr Birgit Osterhoff (International Distributor Coach)
Chairperson : Mrs Norita Salim
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 20th May 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
Sunday, May 10, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Detection of Pelomerase Activity in Saliva from Oral Sqamous Cell Carcinoma Patients"
Presenter : Miss Nurul Hajar Che Ghazali
Chairperson : Miss Suhana Hamzan
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 13th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Detection of Pelomerase Activity in Saliva from Oral Sqamous Cell Carcinoma Patients"
Presenter : Miss Nurul Hajar Che Ghazali
Chairperson : Miss Suhana Hamzan
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 13th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you.
Monday, May 4, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "To freeze or not ?"
Presenter : Dr. Nor Ashikin Mohamed Noor Khan
Chairperson : Mohd Shahir Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 6th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "To freeze or not ?"
Presenter : Dr. Nor Ashikin Mohamed Noor Khan
Chairperson : Mohd Shahir Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 6th May 2009, Wednesday
Time : 12.45 noon
All are welcome. Refreshment will be provided.
Thank you
Monday, April 27, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Comparison of global gene expression of TH2 cytokines stimulated Human a Lung Fibroblasts with stimulated Bronchial
Smooth Muscle Cells"
Presenter : Assoc. Prof Dr. Gabriele R. Anisah Froemming
Title : Identification of host-regulated genes in human lung cancer cell gene expression upon infection with Newcastle
disease virus using microarray analyses.
Presenter : Mr. Mohamed Ros Sidek
Title : Usage of different methods to monitor changes in gene and protein expression of Normal Human Osteoblasts cells
(NHOst) growing on Cytodex 3
Presenter : Mrs. Norita Salim
Title : Molecular Study of Atherosclerotic Plaque development using a multiplexed Gene Expression Assay and Real time PCR
Presenter : Mrs. Maimunah Mustakim
Chairperson : Ms. Zuraini Dollah
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 29th April 2009, Wednesday
Time : 12.30 noon
All are welcome. Refreshment will be provided.
Thank you.
Sunday, April 19, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Mesencyhmal Stem Cell Isolation and Characterization"
Presenter : Dr Chua Kien Hui, UKM-KL
Chairperson : Mrs Norita Salim
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 22th April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
Sunday, April 12, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Biomarker Discovery: SELDI Technology"
Presenter : Ms Sakthi Eswari
Chairperson : Mrs. Norita Salim
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 15th April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Thank you.
Tuesday, April 7, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Effects of Statin Treatment on Bone Markers in Patients with Familial Hypercholesterolaemia"
Presenter : Mrs. Rafezah Razali
Chairperson : Miss Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 8th April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Refreshment will be provided.
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : "Effects of Statin Treatment on Bone Markers in Patients with Familial Hypercholesterolaemia"
Presenter : Mrs. Rafezah Razali
Chairperson : Miss Salina Othman
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 8th April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Refreshment will be provided.
Thank you.
Monday, March 30, 2009
Journal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : ‘Comparison of Weight-Loss Diet with Different Compositions of fat, Protein, and Carbohydrates'
Presenter : Miss Suhana Hamzan
Chairperson : Miss Nurul hajar Ghazali
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 1st April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Refreshment will be provided
Thank you.
I am pleased to invite all of you to our weekly IMMB journal club session that will be held as follow:
Title : ‘Comparison of Weight-Loss Diet with Different Compositions of fat, Protein, and Carbohydrates'
Presenter : Miss Suhana Hamzan
Chairperson : Miss Nurul hajar Ghazali
Venue : IMMB Meeting Room, Level 13, Block 5, S&T Building
Date : 1st April 2009, Wednesday
Time : 12.45 noon
All are welcome.
Refreshment will be provided
Thank you.
Monday, March 23, 2009
Jurnal Club
Dear Dato'/Prof/ Assoc. Prof/ Dr/ colleagues,
Please be informed that there will be an IMMB Journal Club. Below are the details:
Please be informed that there will be an IMMB Journal Club. Below are the details:
Title : "AOE activities and expression levels in the kidney during development of
hypertension in SHR".
Presenter : Prof Dr Harbindarjeet Singh
Chairperson : Mr Mohd Shahir Abdul Rahman
Venue : IMMB Meeting Room, Level 13, Block 5,
Date :
Time :
All are welcome.
Thank you.
Wednesday, March 18, 2009
RESEARCH NEWS OF INTEREST
New Target for Heart Failure Therapy Identified
Drug Discovery & Development -
March 18, 2009
A novel signaling pathway plays a significant role in the production of aldosterone, a hormone that promotes heart failure after a myocardial infarction, according to a study conducted by Thomas Jefferson University researchers.
The findings show that aldosterone production is mediated by a protein called b-arrestin-1. b-arrestin-1 binds to angiotensin II receptors when they are activated by angiotensin II.
Aldosterone is secreted by the adrenal cortex. Its levels are elevated in chronic heart failure, and its presence contributes to morbidity and mortality of the disease. It contributes to heart failure progression and diminished cardiac function after myocardial infarction.
The production of aldosterone was previously thought to be solely the result of the activation of G-proteins, which are also activated when angiotensin II binds to its receptors, according to Anastasios Lymperopoulos, Ph.D., a Post-Doctoral Research Fellow in the Center for Translational Medicine and the George Zallie and Family Laboratory for Cardiovascular Gene Therapy at Jefferson Medical College of Thomas Jefferson University.
http://www.dddmag.com/news-target-for-heart-failure-therapy-031809.aspx
New Target for Heart Failure Therapy Identified
Drug Discovery & Development -
March 18, 2009
A novel signaling pathway plays a significant role in the production of aldosterone, a hormone that promotes heart failure after a myocardial infarction, according to a study conducted by Thomas Jefferson University researchers.
The findings show that aldosterone production is mediated by a protein called b-arrestin-1. b-arrestin-1 binds to angiotensin II receptors when they are activated by angiotensin II.
Aldosterone is secreted by the adrenal cortex. Its levels are elevated in chronic heart failure, and its presence contributes to morbidity and mortality of the disease. It contributes to heart failure progression and diminished cardiac function after myocardial infarction.
The production of aldosterone was previously thought to be solely the result of the activation of G-proteins, which are also activated when angiotensin II binds to its receptors, according to Anastasios Lymperopoulos, Ph.D., a Post-Doctoral Research Fellow in the Center for Translational Medicine and the George Zallie and Family Laboratory for Cardiovascular Gene Therapy at Jefferson Medical College of Thomas Jefferson University.
For more please use the link below
http://www.dddmag.com/news-target-for-heart-failure-therapy-031809.aspx
Health Protection Agency Culture Collection
Cross-Contaminated & Misidentified Cell Lines
The ECACC has published on its webpage various informations concerning cross-contaminated or mis-identified cell lines, how these cell lines can be tested for authencity etc. For the original postings and further information, please follow the following link.
http://www.hpacultures.org.uk/services/celllineidentityverification/misidentifiedcelllines.jsp
For informations on cross-contamination between cell lines found in the literature please read the two articles below. According to the ECACC survey of the scientific literature up to 20% of published work based on the use of human cell lines specifiec lines are suggested or known to be mis-identified1,2. This is not a small number. Therefore everybody who is working with cell lines should look into this matter.
1. MacLeod et al., (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer 83(4) 555-563 (Cell line: ECV304)
2. Drexler HG et al. (2003) False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia, 17(2):416-426.
Cross-Contaminated & Misidentified Cell Lines
The ECACC has published on its webpage various informations concerning cross-contaminated or mis-identified cell lines, how these cell lines can be tested for authencity etc. For the original postings and further information, please follow the following link.
http://www.hpacultures.org.uk/services/celllineidentityverification/misidentifiedcelllines.jsp
For informations on cross-contamination between cell lines found in the literature please read the two articles below. According to the ECACC survey of the scientific literature up to 20% of published work based on the use of human cell lines specifiec lines are suggested or known to be mis-identified1,2. This is not a small number. Therefore everybody who is working with cell lines should look into this matter.
1. MacLeod et al., (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer 83(4) 555-563 (Cell line: ECV304)
2. Drexler HG et al. (2003) False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia, 17(2):416-426.
Tuesday, March 17, 2009
Genome Web Daily News
MicroRNA Profile Predicts Acute Kidney Transplant Rejection
March 17, 2009
By a GenomeWeb staff reporter
NEW YORK (GenomeWeb News) – In a paper appearing online last night in the Proceedings of the National Academy of Sciences, a team of American and French scientists identified a microRNA expression profile that can predict which kidney transplant patients will suffer acute transplant rejection and reduced transplant function.
Using arrays, the researchers identified more than a dozen miRNAs that were differentially expressed in cases of acute kidney transplant rejection compared to stable transplants. They subsequently validated six of these in a larger, independent cohort, linking miRNA levels to both acute rejection and transplant function.
Interestingly, rejection samples and peripheral blood mononuclear cells shared some highly expressed miRNAs, while miRNAs that were under-represented in rejection samples were found at high levels in primary human kidney epithelial cells. Together, the results support the idea that kidney transplant rejection occurs as recipient immune cells enter and attack transplanted tissue. Along with such insights, the team suggested that miRNAs may also serve as markers for kidney transplant or renal allograft status.
"Our studies, in addition to suggesting a cellular basis for the altered intragraft expression of miRNAs, propose that miRNA expression patterns may serve as biomarkers of human renal allograft status," senior author Manikkam Suthanthiran, a nephrology and transplantation researcher at the New York-Presbyterian-Weill Cornell Medical Center, and his colleagues wrote.
Although organ transplantation has become fairly routine, transplant recipients must continue taking immuno-suppressive drugs throughout their lives to prevent transplant rejection. Such rejection occurs when the recipient's immune system recognizes the transplant as foreign and mounts an immune response against it.
Because previous research suggests gene expression — including the expression of genes involved in immunity, cell cycle control, and metabolism — shifts dramatically during transplant rejection, Suthanthiran and his team decided to look at whether they could find miRNA expression changes that heralded acute transplant rejection.
They evaluated 33 transplanted tissue or allograft biopsies, taken from 32 adults who had had kidney transplants — a dozen from 11 individuals with acute rejection and graft dysfunction and 21 from individuals with stable transplanted kidney function.
From these, the team initially evaluated miRNA profiles in three of the acute rejection biopsy samples and four stable transplant samples using an Applied Biosystems TaqMan low-density array human microRNA panel representing 365 mature human miRNAs.
They found roughly 174 miRNAs expressed in each of the samples. But the same miRNAs were not present in all of the biopsies. The researchers identified 17 miRNAs that were expressed at different levels in the acute rejection samples than they were in the stable transplant samples. Of these, ten were under-expressed and seven were over-expressed in the acute rejection samples.
Using modified TaqMan miRNA assays, the researchers confirmed the differential expression of six of the miRNAs in an independent validation set of nine acute rejection and 17 stable transplant biopsy samples. The acute rejection samples showed increased expression of miR-142-5p, miR-155, and miR-223 and decreased expression of miR-10b, miR-30-3p, and let-7c.
All six miRNAs were also linked to the transplanted kidney's function, specifically glomerular filtration rate, though miR-142-5p and miR-10b were most strongly associated with this function.
Of the differentially expressed miRNAs, miR-142-5p and miR-155 seem to be the most promising biomarkers for predicting kidney transplant status. Both predicted acute rejection with 100 percent sensitivity and 90 percent to 95 percent specificity. The other four miRNAs, while diagnostic, predicted rejection less accurately.
When they used real-time quantitative PCR to assess messenger RNA levels in the transplant tissue, the team found that the levels of miR-142-5p, miR-155, and miR-223 correlated with the levels of mRNA for immune genes such as CD30 and CD20. Overall though, miRNA levels appeared to more accurately predict transplant rejection than CD3, CD20 or other mRNA levels tested.
Consistent with the notion that acute transplant rejection involves immune system infiltration of transplanted kidney tissue the team found that rejection miRNA profiles were more similar to normal human peripheral blood mononuclear cells than to primary human kidney epithelial cells.
And miRNAs previously linked to immune system function were among those that were highly expressed in rejection samples whereas miRNAs that appear to contribute to kidney tubule function were down-regulated.
The researchers say even more research is needed to understand just how miRNA profiles are altered in the graft-infiltrating and transplant epithelial cells. But, they added, their results suggest miRNA expression patterns may be a promising tool for predicting and monitoring patients' kidney transplant status.
Invitation to Journal Club Presentation 18 March 2009
Dear all,
Last week we had a presenter from outside of UiTM talking about how we can benefit from NUNC products related to cell culture and etc. To me it was a great presentation and we should think of inviting them again in the near future. Now lets come back to this week presentation. Thank you Prof Justin for his acceptance to be with us. Hope we can share your knowledges from the presentation and you are most welcome again to update us with the latest findings from your research. For all, the graphical form or small poster of invitation is now available. Please distribute to your colleagues. Thank you.
Saturday, March 14, 2009
Asthma
β2-Adrenoceptor signaling is required for the development of an asthma phenotype in a murine model
Nguyen, L. P. et al. Proc. Natl Acad. Sci. USA 106, 2435–2440 (2009) Article PubMed
β2-Adrenergic receptor (β2AR) agonists are used in the management of asthma, but their chronic use is assoicated with loss of disease control. This paper demonstrated the paradoxical result that β2AR signalling — by constitutively active receptors — is required for the asthma phenotype in animal models. A β2AR inverse agonist attenuated asthmatic symptoms, highlighting the potential of inverse agonists in this condition.
β2-Adrenoceptor signaling is required for the development of an asthma phenotype in a murine model
Nguyen, L. P. et al. Proc. Natl Acad. Sci. USA 106, 2435–2440 (2009) Article PubMed
β2-Adrenergic receptor (β2AR) agonists are used in the management of asthma, but their chronic use is assoicated with loss of disease control. This paper demonstrated the paradoxical result that β2AR signalling — by constitutively active receptors — is required for the asthma phenotype in animal models. A β2AR inverse agonist attenuated asthmatic symptoms, highlighting the potential of inverse agonists in this condition.
The Scientist
By Steven Wiley
-------------------------------------------------------------------------------------------------
The Problem of Perception
http://www.the-scientist.com/article/display/55467/
Your interpretation of results depends on more than just the results.
Another group felt that our computer model was a poor substitute for their own scientific intuition regarding what was happening.
There is a common perception among young students that the surest path to resolving scientific controversies is to design a clever experiment, one that will definitively resolve conflicting hypotheses. However, I have found that most scientific controversies do not revolve around specific experimental data, but instead are disputes over data interpretation.
Data interpretations depend on a scientist's underlying assumptions and worldview. For example, a molecular biologist might think of protein expression as an outcome of mRNA levels, whereas a biochemist might think in terms of synthetic and degradation rates. Both are right, of course, but each might expect different reasons for a change in the amount of a protein. Our perspective and assumptions regarding how living systems work defines us as biologists, which is why arguments over interpretations can get so nasty. If another scientist disputes the validity of your viewpoint, it can impact your reputation as well as your ego.
I experienced a dispute over data interpretation in the late 1980's that turned somewhat contentious. My laboratory was investigating the role of tyrosine kinase activity in the endocytosis of the EGF receptor in collaboration with Gordon Gill at the University of California, San Diego. By using a series of kinetic measurements, we concluded that kinase activity was necessary for ligand-induced endocytosis and down-regulation, whereas a competing group thought that it was not. What was interesting about the dispute was that the primary data gathered by both groups were essentially identical. The main difference was in how the data were interpreted.
We were calculating receptor endocytosis rates on a per receptor basis whereas the other group used a per cell basis. In the case of receptors with kinase activity, EGF binding accelerated endocytosis 10-fold, which caused cell-surface receptors to drop 10-fold. Thus, the net rate of endocytosis per-cell (rate x receptors) did not change in response to kinase activity, making it appear to have no effect on receptor internalization.
It was hard to convince the other group that differences in a simple rate calculation would make a big difference in what they concluded from the data. However, we were working from a computational model of endocytosis that allowed us to try out different sets of assumptions and see how they would affect the system's behavior. The other group felt that our computer model was a poor substitute for their own scientific intuition regarding what was happening. As they stated at the time, "(Our) results are in agreement with the results of Chen et al. (Cell, 59:33–43,1989), but in conflict with their interpretation. By applying a simple mathematical model (J Cell Biol, 107:801–10, 1988), the lack of down-regulation of kinase-negative mutants was misinterpreted as an indication for reduced endocytosis." 1
Over time, our view prevailed and it is now generally accepted that the kinase activity of the EGF receptor is required for ligand-induced endocytosis. 2 Interestingly, our view was vindicated not because people came to accept our use of computational modeling, but because our hypothesis was more successful in predicting subsequent experimental results. Scientists don't generally care about who is right or who is wrong in a dispute. They want a conclusion that can help predict their own experimental outcomes. Science is built brick by brick from ideas and concepts that can lead to the next successful series of experiments and concepts. If an idea doesn't support the next brick, it is discarded. It's natural selection in science.
Scientific disputes seem inevitable in any career, but mine gave me a keen appreciation of the need for caution in accepting simple interpretations of the behavior of complex systems. In science, we do not gather facts. We make observations. Our interpretation of observations is only as good as our assumptions and conceptual frameworks.
The ability of a simple computer model to correctly interpret a seemingly non-intuitive result was also quite revealing. It convinced me that as biology becomes even more complex, we will need computational models even more to help us out. They can not only let us see past our inherent interpretational biases, but can also be used to design experiments to test our concepts—a foundation for building a truly predictive biology.
Steven Wiley is a Pacific Northwest National Laboratory Fellow and director of PNNL's Biomolecular Systems Initiative.
References
1. A. Ullrich, and J. Schlessinger, "Signal transduction by receptors with tyrosine kinase activity," Cell, 61:203–12, 1990.
2. A. Sorkin, and L.K. Goh, "Endocytosis and intracellular trafficking of ErbBs," Experimental Cell Res, 314:3093–106, 2008.
By Steven Wiley
-------------------------------------------------------------------------------------------------
The Problem of Perception
http://www.the-scientist.com/article/display/55467/
Your interpretation of results depends on more than just the results.
Another group felt that our computer model was a poor substitute for their own scientific intuition regarding what was happening.
There is a common perception among young students that the surest path to resolving scientific controversies is to design a clever experiment, one that will definitively resolve conflicting hypotheses. However, I have found that most scientific controversies do not revolve around specific experimental data, but instead are disputes over data interpretation.
Data interpretations depend on a scientist's underlying assumptions and worldview. For example, a molecular biologist might think of protein expression as an outcome of mRNA levels, whereas a biochemist might think in terms of synthetic and degradation rates. Both are right, of course, but each might expect different reasons for a change in the amount of a protein. Our perspective and assumptions regarding how living systems work defines us as biologists, which is why arguments over interpretations can get so nasty. If another scientist disputes the validity of your viewpoint, it can impact your reputation as well as your ego.
I experienced a dispute over data interpretation in the late 1980's that turned somewhat contentious. My laboratory was investigating the role of tyrosine kinase activity in the endocytosis of the EGF receptor in collaboration with Gordon Gill at the University of California, San Diego. By using a series of kinetic measurements, we concluded that kinase activity was necessary for ligand-induced endocytosis and down-regulation, whereas a competing group thought that it was not. What was interesting about the dispute was that the primary data gathered by both groups were essentially identical. The main difference was in how the data were interpreted.
We were calculating receptor endocytosis rates on a per receptor basis whereas the other group used a per cell basis. In the case of receptors with kinase activity, EGF binding accelerated endocytosis 10-fold, which caused cell-surface receptors to drop 10-fold. Thus, the net rate of endocytosis per-cell (rate x receptors) did not change in response to kinase activity, making it appear to have no effect on receptor internalization.
It was hard to convince the other group that differences in a simple rate calculation would make a big difference in what they concluded from the data. However, we were working from a computational model of endocytosis that allowed us to try out different sets of assumptions and see how they would affect the system's behavior. The other group felt that our computer model was a poor substitute for their own scientific intuition regarding what was happening. As they stated at the time, "(Our) results are in agreement with the results of Chen et al. (Cell, 59:33–43,1989), but in conflict with their interpretation. By applying a simple mathematical model (J Cell Biol, 107:801–10, 1988), the lack of down-regulation of kinase-negative mutants was misinterpreted as an indication for reduced endocytosis." 1
Over time, our view prevailed and it is now generally accepted that the kinase activity of the EGF receptor is required for ligand-induced endocytosis. 2 Interestingly, our view was vindicated not because people came to accept our use of computational modeling, but because our hypothesis was more successful in predicting subsequent experimental results. Scientists don't generally care about who is right or who is wrong in a dispute. They want a conclusion that can help predict their own experimental outcomes. Science is built brick by brick from ideas and concepts that can lead to the next successful series of experiments and concepts. If an idea doesn't support the next brick, it is discarded. It's natural selection in science.
Scientific disputes seem inevitable in any career, but mine gave me a keen appreciation of the need for caution in accepting simple interpretations of the behavior of complex systems. In science, we do not gather facts. We make observations. Our interpretation of observations is only as good as our assumptions and conceptual frameworks.
The ability of a simple computer model to correctly interpret a seemingly non-intuitive result was also quite revealing. It convinced me that as biology becomes even more complex, we will need computational models even more to help us out. They can not only let us see past our inherent interpretational biases, but can also be used to design experiments to test our concepts—a foundation for building a truly predictive biology.
Steven Wiley is a Pacific Northwest National Laboratory Fellow and director of PNNL's Biomolecular Systems Initiative.
References
1. A. Ullrich, and J. Schlessinger, "Signal transduction by receptors with tyrosine kinase activity," Cell, 61:203–12, 1990.
2. A. Sorkin, and L.K. Goh, "Endocytosis and intracellular trafficking of ErbBs," Experimental Cell Res, 314:3093–106, 2008.
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